Measure RNA expression in millions of single cells using standard flow cytometers.
Discover the comprehensive benefits of Dahlia's RNA Flow Assay technology designed to elevate your research.
Analyze gene expression at the individual cell level with high fidelity and precision. Target the ~90% of proteins that lack high-quality flow cytometry antibodies by detecting their RNA transcripts instead.
Streamlined protocols that save time and reduce hands-on steps without compromising data quality. Complete the entire workflow in less than 7 hours.
Works seamlessly with a wide range of sample types, including PBMCs, dissociated tissues, and cell lines.
Tailor your assays with probe designs specifically engineered for your unique RNA targets. Currently available in 3-color configurations.
Proprietary amplification technology ensures clear, distinguishable signals even for low-abundance transcripts. Validated over a 3-log linear range.
Enrich cells using RNA markers that lack high-quality flow cytometry antibodies for downstream genomics analysis.
Discover how Dahlia RNA Flow Assays can accelerate your research.
Target non-coding RNA or the ~90% of proteins that lack high-quality flow cytometry antibodies by detecting its mRNA proxy.
Enrich rare cell populations based on specific RNA markers prior to downstream sequencing.
Rapidly validate single-cell RNA sequencing discoveries with high-throughput flow cytometry.
Supplement existing antibody flow panels with RNA targets of interest.
Compatible with your existing antibody panels, 96-well plate formats, and standard fluorescence-based flow cytometers.
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Explore the robust performance and reliability of our RNA Flow Assays across various applications.
Simultaneous detection of multiple cytokine RNA targets in activated versus control peripheral blood mononuclear cells (PBMCs).
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Co-detection of CD3 surface proteine expression and CD3 RNA.
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Dahlia RNA Flow Assays can be designed to discriminate fusion RNA and splice variants.
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Level of fluorescence signal correlates to expected level of RNA expression, with higher levels of expression yielding higher MFI values
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Detection of CD3E RNA in peripheral mononuclear cells (PBMCs) across multiple replicates.
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To get started, you simply need to order RNA Flow Probe Sets and RNA Flow Assay Reagent Kits.
Target specific
RNA Flow Probe Sets
Multiplex up to 3 RNA targets (Cy5, Cy3, FITC)
Designed against any RNA target with proprietary pipeline
Wet lab validated to ensure high sensitivity and specificity
3 week turn around time
UNIVERSAL
RNA Flow Assay Kits
All buffers and reagents needed
Optional antibody co-staining protocol
Sizes: Small (25 tests) and Large (100 tests)
Resources
Explore the latest protocols, experimental planning tips, and FAQs to get the most out of your assays.
Get in Touch
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